Soheila Abachi Hokmabadinazhad
M.Sc, Institute of Nutrition and Functional Foods, Universite Laval, Canada
Title: Identification of gastrointestinal digestion stable antihypertensive fish peptides from Atlantic mackerel (Scomber scombrus)
Biography
Biography: Soheila Abachi Hokmabadinazhad
Abstract
High blood pressure has been recognized as one of the main risk factors of cardiometabolic syndrome. In addition, there is a link between the occurrence of insulin resistance and hypertension consequently initiating type-2 diabetes. Hypertension is medicated by various classes of synthetic drugs; however, adverse effects have repeatedly been reported. To avoid and reduce side effects, natural alternatives such as marine biopeptides have been shown effective in the treatment and prevention of hypertension and its associated complications. The aim of present work was to fractionate and identify antihypertensive peptides by means of measuring the angiotensin-converting enzyme (ACE) inhibition capacity of fractions combined with UPLC-MS identification. Fractions were produced using different techniques of solid phase extraction (SPE), pressure-driven ultrafiltration (UF) and electrodialysis with UF membrane (EDUF). According to our results the hydrophobic fraction of SPE with the IC50 value of 5 μg of protein was the most potent anti-ACE product. Findings of the study suggest that separation technique plays an important role in the isolation efficiency of antihypertensive biopeptides since the IC50 values of EDUF and UF fractions were higher and or insignificantly different from the IC50 value of the hydrolysate, 8.8 ug of protein. Importantly, the hydrophobic fraction’s activity was retained through in-vitro gastrointestinal digestion system. In conclusion, polarity regardless of other properties, was the most important factor for anti-ACE activity of the fraction. In addition, the presence of leucine at either of the extremities and or leucine rich motifs could well explain the hypotensive effect of the active fractions.